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. 2021 May 28;22(11):5772. doi: 10.3390/ijms22115772

Figure 3.

Figure 3

Effect of priming on the production of critical immunomodulatory factors in tonsil-derived mesenchymal stem cell (T-MSC). (A) Comparison of soluble factors expression, including IDO, COX-2, HO-1, TGF-β1, IL-10, and iNOS between T-MSCCon and T-MSCPrimed using RT-PCR (* p < 0.05, ** p < 0.001, *** p = 0.0008). (B) Comparison of costimulatory molecule expression, such as PD-L1, ICOSL, and iNOS between T-MSCCon and T-MSCPrimed using flow cytometry. (C) IDO inductivity of T-MSCPrimed is confirmed by ELISA (*** p = 0.0004). (D) Protein expression of CD40 and IDO is confirmed by flow cytometry between T-MSCCon and T-MSCPrimed. T-MSCCon, tonsil-derived mesenchymal stem cell with the addition of vehicle control; T-MSCPrimed, tonsil-derived mesenchymal stem cell primed with TNF-α and IFN-γ; IDO, indoleamine 2,3-dioxygenase quantification; HO-1, heme oxygenase; PD-L1, programmed death-ligand 1; ICOSL, inducible T cell costimulator ligand.