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. 2021 May 28;22(11):5772. doi: 10.3390/ijms22115772

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Indoleamine 2,3-dioxygenase quantification (IDO) and CD40 are key molecules on the immunomodulatory effect of the primed tonsil-derived mesenchymal stem cells (T-MSCs). (A) Expression of TNF-α, IFN-γ, and IL-4 in CD4⁺ T cells according to the different groups (control, PHA, T-MSC^Con, T-MSC^Primed and inhibitor IDO) (B) Expression of TNF-α, IFN-γ, and IL-4 in CD4⁺ T cells according to the different groups (control, TCR, T-MSC^Con, T-MSC^Primed, and Anti-CD40Ab) (C) Expression level of transcription factors of Th1 (T-bet) and Th2 (GATA3) both in the co-cultures of T-MSCs and CD4⁺ T cells according to the different groups (control, TCR, T-MSC^Con, T-MSC^Primed, and Anti-CD40Ab). Dot blots are representative of each independent experiment. The graphic data is the ± SEM from each independent experiment. * p < 0.05 and ** p < 0.01. T-MSC^Con, tonsil-derived mesenchymal stem cell with the addition of vehicle control; T-MSC^Primed, tonsil-derived mesenchymal stem cell primed with TNF-α and IFN-γ; Anti-CD40Ab, Neutralizing anti-CD40 antibodies.

Indoleamine 2,3-dioxygenase quantification (IDO) and CD40 are key molecules on the immunomodulatory effect of the primed tonsil-derived mesenchymal stem cells (T-MSCs). (A) Expression of TNF-α, IFN-γ, and IL-4 in CD4⁺ T cells according to the different groups (control, PHA, T-MSC^Con, T-MSC^Primed and inhibitor IDO) (B) Expression of TNF-α, IFN-γ, and IL-4 in CD4⁺ T cells according to the different groups (control, TCR, T-MSC^Con, T-MSC^Primed, and Anti-CD40Ab) (C) Expression level of transcription factors of Th1 (T-bet) and Th2 (GATA3) both in the co-cultures of T-MSCs and CD4⁺ T cells according to the different groups (control, TCR, T-MSC^Con, T-MSC^Primed, and Anti-CD40Ab). Dot blots are representative of each independent experiment. The graphic data is the ± SEM from each independent experiment. * p < 0.05 and ** p < 0.01. T-MSC^Con, tonsil-derived mesenchymal stem cell with the addition of vehicle control; T-MSC^Primed, tonsil-derived mesenchymal stem cell primed with TNF-α and IFN-γ; Anti-CD40Ab, Neutralizing anti-CD40 antibodies.