Table 1.
Biochemical parameters of the blood plasma of mice fed experimental diets, M ± S.E.M *.
| Genotype | DBA/2J | DBCB | Factor *** | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Group No | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | |
| Diet | Control | HFCD | HFCD + Tyr | HRCD + Trp | Control | HFCD | HFCD + Tyr | HRCD + Trp | |
| Number of Animals | 8 | 7 | 8 | 8 | 7 | 4 | 7 | 7 | |
| Glucose, mmol/L | 12.8 ± 0.7 2–8* | 16.2 ± 0.9 1 | 17.8 ± 0.8 1 | 19.8 ± 1.9 1 | 20.6 ± 1.8 1 | 22.1 ± 2.5 1 | 20.2 ± 1.8 1 | 20.2 ± 0.7 1 | G |
| Protein, g/L | 52.0 ± 2.5 3,4 | 52.9 ± 2.0 3,4 | 61.1 ± 1.2 1,2 | 59.8 ± 2.4 1,2 | 58.0 ± 3.6 | 56.1 ± 4.3 | 61.5 ± 1.2 | 60.1 ± 1.0 | Tyr |
| Albumin, g/L | 25.7 ± 1.2 3,4 | 25.0 ± 1.9 3 | 30.1 ± 0.7 1,2 | 29.6 ± 1.4 1 | 31.0 ± 1.2 | 29.7 ± 2.0 | 32.1 ± 0.6 | 32.7 ± 0.5 | Tyr, Trp |
| Urea, mmol/L | 5.15 ± 0.45 3,4 | 5.18 ± 0.34 3,4 | 7.27 ± 0.43 1,2 | 6.54 ± 0.32 1,2 | 6.63 ± 0.42 | 5.57 ± 0.32 7,8 | 7.03 ± 0.46 6 | 6.59 ± 0.22 6 | G ****, Tyr, Trp |
| Calcium, mmol/L | 1.86 ± 0.09 3,4 | 1.86 ± 0.10 3,4 | 2.35 ± 0.12 1,2 | 2.34 ± 0.14 1,2 | 2.15 ± 0.06 | 1.95 ± 0.19 8 | 2.30 ± 0.08 | 2.26 ± 0.06 6 | Tyr, Trp |
| Phosphorus, mmol/L | 2.51 ± 0.19 4 | 2.95 ± 0.19 | 3.06 ± 0.26 | 3.72 ± 0.32 1 | 3.30 ± 0.20 | 3.03 ± 0.36 | 3.14 ± 0.23 | 3.37 ± 0.19 | Trp |
| AlAT, U/mL | 22.0 ± 3.8 3 | 27.5 ± 11.1 | 50.9 ± 13.4 1 | 35.3 ± 8.6 | 19.6 ± 4.9 6 | 62.8 ± 20.5 5 | 26.9 ± 6.2 | 47.7 ± 16.2 | D, G × Tyr |
| AsAT, U/mL | 220 ± 43 | 201 ± 39 | 205 ± 29 | 258 ± 66 | 180 ± 35 | 454 ± 253 | 191 ± 39 | 220 ± 38 | G × D |
| AsAT/AlAT | 11.2 ± 2.0 3 | 10.6 ± 2.5 3 | 5.0 ± 0.8 1,2 | 9.9 ± 2.6 | 12.5 ± 5.1 | 10.1 ± 4.6 | 5.1 ± 0.3 | 12.3 ± 4.7 | Tyr |
| KPK kU/mL | 5.7 ± 1.7 | 5.9 ± 1.6 | 5.9 ± 0.9 | 7.1 ± 1.9 | 5.1 ± 0.7 | 10.7 ± 3.7 7 | 3.6 ± 1.0 6 | 5.6 ± 1.4 | Tyr |
| Lipase U/mL | 129 ± 13 | 110 ± 4 4 | 120 ± 3 | 138 ± 9 2 | 119 ± 15 | 111 ± 7 8 | 148 ± 20 | 140 ± 8 6 | Trp |
| GPO mmol/min/ mg protein ** |
0.71 ± 0.02 2,3,4 | 0.39 ± 0.03 1 | 0.40 ± 0.03 1 | 0.46 ± 0.01 1 | 0.68 ± 0.03 6,7 | 0.44 ± 0.03 5,8 | 0.49 ± 0.06 5 | 0.60 ± 0.02 6 | G, D, Trp |
* Superscripts are group numbers, the difference with which is pairwise significant, p < 0.05, non-parametric Mann–Whitney test. ** In erythrocytes, the number of animals in groups of 6. *** Factors significantly influencing the distribution of indicator presented between groups of animals, p < 0.05, ANOVA, such as genotype of the animals (G), diet type (D), and amino acid added (Tyr, Trp). **** Only in experiment with Trp. See all abbreviations in Materials and Methods section.