Table 5.
The effect of solvent type and method of extraction on the yield of triterpene aglycones [µg/g DW] in Polyscias filicifolia TSE regenerants cultured in vitro.
| Plant Material | Oleanolic Acid | Ursolic Acid | Hederagenin | Ratio OA to HE |
|---|---|---|---|---|
| Nonembryogenic callus | 18.62 ± 0.28 a | not detected | 9.53 ± 0.50 a | (2:1) |
| Embryogenic callus | 17.64 ± 0.15 b | not detected | 9.81 ± 0.33 b | (2:1) |
| TSE regenerants | 439.72 ± 0.64 a,b,c | 19.07 ± 0.21 a | 111.85 ± 0.48 a,b,c | (4:1) |
| TSE plants ex vitro | 47.10 ± 0.87 a,b,c | 8.82 ± 0.55 a | 16.06 ± 0.39 a,b,c | (3:1) |
| Donor plant | 55.37 ± 0.94 a,b,c | 7.90 ± 0.41 a | 17.02 ± 0.58 a,b,c | (3:1) |
Data of oleanolic acid (OA), ursolic acid (UA) and hederagenin (HE) were expressed by mean ± SD values. Mean values denoted with same the same letter within groups were significantly different at p ˂ 0.05 according to Tukey test. OA: oleanolic acid; UA: ursolic acid; HE: Hederagenin; SD: Standard deviation; DW: Dry weight.