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. 2021 May 27;26(11):3210. doi: 10.3390/molecules26113210

Figure 1.

Figure 1

Measurement of ROS generation in SH-SY5Y differentiated cells after RB extract treatment. Protective effect of RB on total ROS generation induced by t-BHP treatment. SH-SY5Y differentiated cells were treated with increasing concentrations (1000, 500, 250, 125 and 62 µM) of RB for 3 h and the ROS generation was measured using the DCFH-DA assay. Figure shows the fold change in the percentage of mean fluorescence intensity as compared to the group treated with 500 µM t-BHP (maximum ROS production). Data represents the mean ± standard deviation of three independent experiments; different letters (a, b) indicate significant differences (p < 0.05). Linear effect of increasing level of RB on ROS generation.