Figure 5.

Measurement of ROS generation in SH-SY5Y differentiated cells after cyanidin-3-glucoside treatment. Protective effect of cyanidin on total ROS generation induced by t-BHP treatment. SH-SY5Y differentiated cells were treated with decreasing concentrations of cyanidin-3-glucoside (from 10 to 0.1 µM) for 3 h and the ROS generation was measured using the DCFH-DA assay. Figure shows the fold change in the percentage of mean fluorescence intensity as compared to the group treated with 500 µM t-BHP (maximum ROS production). Data represent the mean ± standard deviation of three independent experiments. Different letters (a, b) indicate significant differences (p < 0.05).