Figure 2.

Influence of NMRT on HDAC, COX2 and CDK4 phosphorylation. NMRT induced changes in the expression of HDAC4 and COX2 (A,D) and the activity (R.L.U., relative luminescence units) of HDAC I/II (B) in control (HC) and OA cells are shown. (A) summarizes the NMRT induced changes in expression of HC and OA cells (untreated cells functioned as calibrators). Concentration response curve for the inhibition of HDACI/II activity by trichostatin (nM) with or without NMRT treatment in (C) (n = 3). The box-plot in D represents the difference in expression of HDAC4/COX2 of OA cells (OA) or OA cells treated with NMRT (OA^NMRT) compared to HC (HC untreated functioned as calibrator; HC/OA; HC/OA^NMRT); treated OA cells were further compared with NMRT treated HC cells leading to HC^NMRT/OA^NMRT (HC^NMRT cells as calibrator); n = 4 and each experiment was executed in duplicates. Significant changes compared to the respective calibrator are given: *: p < 0.021 and **: p = 0.004. CDK4 phosphorylation by western blotting and phospho-CDK4 normalization to CDK4 is shown (E). The statistical significance for the bar charts and inhibitor study was evaluated by using the Student’s t-test while the differences in median values represented by the box-plots were calculated via the Mann–Whitney Rank Sum test; *: p < 0,05; **: p < 0,01.