Figure 3.

Inhibition of ICAM-1 binding to bead-immobilized PF11_0521 DBL2β3 domain by twenty-four mixtures (out of 50) with R¹ (A) and forty mixtures (out of 70) with R² (B) groups in TPI-2103 with highest binding inhibiting activity. Mixtures were assayed at concentration 2.5 μg/mL. We measured 100% binding in the absence of compounds, using binding buffer supplemented with the same concentration of DMF as samples with compounds. All groups selected for the synthesis of single compounds library (TPI-2648) are indicated by red color (active) and by black color (inactive). List of building blocks for R1 and R2 groups is also included in Supplementary Materials.