KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-PPARgamma (E-8) | Santa Cruz Biotechnology | Cat# sc-7273 |
| Rabbit polyclonal anti-PPARG (81B8) | Cell Signaling | Cat# 2443 |
| Mouse monoclonal anti-p21 (F5) | Santa Cruz Biotechnology | Cat# sc-6246 |
| Rabbit polyclonal anti-C/EBPα | Santa Cruz Biotechnology | Cat# sc61 |
| Goat polyclonal anti-FABP4 | R & D Systems | Cat# AF1443 |
| Mouse monoclonal anti-Adiponectin | Abcam | Cat# ab22554 |
| Goat polyclonal anti-Glut4 | Santa Cruz Biotechnology | Cat# sc-1608 |
| Goat anti- Rabbit IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 514 | Invitrogen | Cat# A31558 |
| Goat anti- Rabbit IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 647 | Invitrogen | Cat# A21244 |
| Goat anti- Mouse IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 594 | Invitrogen | Cat# A11032 |
| Goat anti- Mouse IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 647 | Invitrogen | Cat# A21235 |
| Donkey anti- Mouse IgG (H+L) cross-adsorbed secondary antibody, Alexa Fluor 647 | Invitrogen | Cat# A31571 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| IBMX | Sigma-Aldrich | Cat# 7018 |
| Dexamethasone | Sigma-Aldrich | Cat# D1756 |
| Insulin | Sigma-Aldrich | Cat# I6634 |
| Saponin | Sigma-Aldrich | Cat# 47036 |
| Bovine Serum Albumin | Sigma-Aldrich | Cat# 7906 |
| Rosiglitazone | Cayman Chemical Company | Cat# 7906 |
| Trimethoprim (TMP) | Cayman Chemical Company | Cat# 16473 |
| HCS LipidTOX Deep Red neutral lipid stain | Thermo Fisher Scientific | Cat# H34477 |
| Experimental Models: Cell Lines | ||
| OP9 mouse stromal | Wolins et al., 2006 | N/A |
| citrine(YFP)-PPARG OP9 cells | Bahrami*,Zhao*, et al., 2018 | N/A |
| Dual reporter citrine-PPARG and APC/C-reporter-mcherry (RFP) OP9 cells | This paper | N/A |
| Triple reporter citrine-PPARG, APC/C-reporter-mcherry (RFP), CDK2 activity sensor (CFP) OP9 cells | This paper | N/A |
| Dual reporter citrine-PPARG and CRL4-Cdt2-mcherry (RFP) OP9 cells | This paper | N/A |
| siRNA Sequences | ||
| See Table S1 | N/A | |
| Recombinant DNA | ||
| Plasmid: CSII-EF1-H2B-mTurquoise | Gift from Tobias Meyer Laboratory | N/A |
| Plasmid: CSII-EF1-H2B-iRFP670 | This paper | N/A |
| Plasmid: CSII-EF-MCS-mCherry-Geminin1to110 | Gift from Tobias Meyer Laboratory | N/A |
| Plasmid: CSII-EF-MCS-mCerulean-Geminin1to110 | Gift from Tobias Meyer Laboratory | N/A |
| Plasmid: pLV-EF1a-hDHB-mTurquoise-Blast | Gift from Tobias Meyer Laboratory | N/A |
| Plasmid: pCru5-6.10-TCT-ACC-DHFR-p21-IRES-puro | Gift from Tobias Meyer Laboratory | N/A |
| Plasmid: pCru5-6.10-TCT-ACC-DHFR-mChy-IRES-puro | Gift from Tobias Meyer Laboratory | N/A |
| Plasmid: iRFP670-CRL4-Cdt2 | This paper | N/A |