Table 3.
Bands and assignments of molecular spectral components extracted from MCR analysis of the Raman image of the tissue HAp calcification shown in Figure 2. Assignments were based on the literature [40,41,42,43].
| Component 3: Lipid | Component 4: Polysaccharides and Proteins | Component 5: Cytochrome C | |||
|---|---|---|---|---|---|
| Band/cm−1 | Assignment | Band/cm−1 | Assignment | Band/cm−1 | Assignment |
| 436 | Cholesterol, cholesterol ester | 424 | Polysaccharide | 749 | Cytochrome C |
| 597 | Phosphatidylinositol | 496 | Glycogen | 1002 | Phenylalanine |
| 624 | Cholesterol ester | 850 | Polysaccharide | 1128 | Cytochrome C |
| 721 | Phosphatidylcholine, Sphingomyelin | 1002 | Phenylalanine | 1174 | CH tyrosine phenylalanine |
| 1081 | Phosphatidylcholine, membrane lipids | 1062 | C-C carbohydrates | 1232 | Amide III |
| 1133 | Membrane lipids (PC, PE, SM) | 1126 | Disaccharides, glucose, sucrose, C-O stretching carbohydrates | 1312 | Cytochrome C |
| 1300 | Membrane lipids (PC, PE) | 1205 | Amide III proteins | 1339 | tryptophan |
| 1314 | CH3CH2 twisting of lipid | 1270 | Amide III proteins | 1449 | C-H proteins |
| 1450 | CH (lipids and proteins) | 1297 | CH2 deformation | 1587 | Cytochrome C |
| 1653 | C=C lipids, proteins | 1322 | CH deformation proteins | 1658 | Amide I proteins |
| 1342 | CH deformation protein and carbohydrates | ||||
| 1447 | CH2 deformation proteins | ||||
| 1553 | Tryptophan, Amide II | ||||
| 1603 | Phenylalanine | ||||
| 1656 | Amide I proteins | ||||