Figure 3.

Glycogen content changes in breast cancer tissue are associated with obesity. Periodic Acid-Schiff (PAS) staining and light microscopy showing glycogen localization patterns in benign and malignant tumor tissues. Glycogen (purple staining) localization in breast cancer cells in malignant tumor tissue and breast epithelial cells in benign tumor tissue of normal-weight and overweight/obese premenopausal women. For each sample, PAS staining is presented next to the serial section pretreated with diastase, an enzyme that catalyzes glycogen hydrolysis. Magnification: ×100, orig. Scale bar: 20 μm.