Table 2.
Characterization of the saponins tentatively identified by LC-MS (+/−) of the ethanolic extracts of different organs from A. acutifolius.
| Molecular Ion (m/z) | Ion Fragmentation | ||||||
|---|---|---|---|---|---|---|---|
| Saponin | Rt a (min) | Formula | [M − H]-Experimental | [M − H]- Theoretical |
Error (ppm) |
Neg Mode | Pos Mode |
| HTSAP-1 | 24.9 | C50H83O23 | 1051.5331 | 1051.5284 | 4.4 | b Pen919-c Hex757-Hex595-Hex433 | [-Na-H2O]1035-Hex873-Hex711-Pen579-Hex417 e |
| ACSAP-1 | 27.8 | C50H83O23 | 1051.5331 | 1051.5306 | 2.4 | Pen919-Hex757-Hex595-Hex433 | [-Na-H2O]1035-Hex873-Hex711-Pen579-Hex417 |
| HTSAP-3 | 29.6 | C49H83O22 | 1021.5225 | 1021.5212 | 1.2 | Pen889-Pen757-Hex595-Hex433 | [-Na-H2O]1005-Pen873-Hex711-Pen579-Hex417 |
| HTSAP-6 | 32.9 | C50H83O23 | 1035.5381 | 1035.5383 | 0.1 | Pen903-d DoHex757-Hex595-Hex433 e | [-Na-H2O]1019-DoHex873-Pen741-Hex579-Hex417 |
| ACSAP-2 | 42.2 | C44H71O18 | 887.4646 | 887.4601 | 5 | Pen755-Hex593-Hex431 e | [-Na-H2O]871-Hex709-Pen577-Hex415 e |
| ACSAP-3 | 44.1 | C43H69O17 | 857.4540 | 857.4503 | 4.3 | Pen725-Pen593-Hex431 | [-Na-H2O]841-Pen709-Pen577-Hex415 |
a Rt: retention time. b Pen: Pentose. c Hex: Hexose. d DoHex: Deoxyhexose. e 433,431,417,415: Genins.