Figure 1.

Chemical/enzymatic methods for lnRNAs structure analysis. (1) Probing lnRNAs by chemical probes which form covalent adducts, or by enzymes; (2) reverse transcription of modified or fragmented lnRNAs; (a) ‘stop’ event (blue dot) occurs when RT (reverse transcriptase) stalls or dissociates before adduct, resulting in a truncated fragment; (b) ‘mutation’ event occurs when RT proceeds through the adduct introducing a mutation; (3) detection using mutation or fractionation readout approaches. This is an original figure created in BioRender.com (accessed on 25 May 2020).