Figure 2.

Methods for detection of RNA-RNA interactions. (A) Crosslinking RNAs directly, (B) crosslinking RNAs in the presence of RNA binding protein (RBP). PARIS, SPLASH, LIGR-seq include crosslinking RNAs with psoralen, digestion with RNase, proximity ligation and sequencing. In hiCLIP after RNA digestion, adjacent RNAs ends are ligated using a linker. In MARIO, RNA binding protein (RBP) is biotinylated, enabling immobilisation of RNA–RBP followed by digestion of RNAs ends and their ligation with added biotinylated linker; the tagged RNA–protein complexes are then purified and sequenced. This is an original figure.