Figure 4.

Chaperone-mediated autophagy. Substrate proteins with KFERQ motif are identified by Hsc70 (Heat Shock Cognate protein 70). Next, the recognized substrates are transported by Hsc70 and its co-chaperones on the lysosome surface. Delivered proteins bind to the LAMP2A (Lysosome-Associated Membrane Protein type 2A) and LAMP2A-protein complex is formed. At the final stage of the CMA process, substrate proteins are transported into the lysosome lumen and their degradation by hydrolytic enzyme occurs.