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. 2021 Jun 12;9:137. doi: 10.1186/s40168-021-01078-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Phylogenetic tree of order Methanomassiliicoccales and analysis of FE-enriched genomes. a Maximum-likelihood tree of the 82 Methanomassiliicoccales genomes constructed using PhyloPhlAn [30]. Stars indicate the 66 MAGs reconstructed in this study. The colored bars in the outer layer indicate the GIT regions from which these genomes were obtained these genomes. RUM, rumen; RET, reticulum; OMA, omasum; ABO, abomasum; DUO, duodenum; JEJ, jejunum; ILE, ileum; CEC, cecum; COL, colon; REC, rectum. b Heatmaps showing counts of selected CAZyme-encoding genes in the 410 differentially enriched genomes identified between the high- and low-FE groups. The most prevalent assigned genera in these genomes are denoted on the left