Table 1.

A summary of cell studies that explicitly demonstrate the recruitment (or lack) of TDP-43 to stress granules identified by one or more of the key SG markers, G3BP, TIA1, TIAR, HuR, eIF3 and FMRP. Only papers that explicitly assessed TDP-43 recruitment to granules are included, even if this was not the main thrust of the paper. Any publications which failed to show clear SG formation using a core SG marker, irrespective of their TDP-43 findings, are not included. With rare exceptions, the majority of findings support the hypothesis that irrespective of stress or cell type, if robust SGs form, TDP-43 is recruited to them. Endog = endogenous; Exog = exogenous, WT = wild type. † TDP-43 antibody-dependent.

Cell Type	Stressor	SG Marker	TDP Recruitment to SGs	Type of TDP	Ref.
Fibroblasts	Arsenite	TIAR	chronic (30 h) only	Endog WT and A382T	[90]
		HuR/TIA1	N	Endog WT and A382T	[91]
Fibroblasts	H2O2	TIAR	Y	Endog WT, A382T, G294V	[92]
COS-7	4-Hydroxynonenal	TIAR	Rare but persistent	Endog WT	[93]
COS-7	Arsenite	G3BP1/TIAR/	Y	Exog WT-YFP	[94]
		eIF3
COS-7	Heat Shock	G3BP1	Y	Exog WT-GFP	[94]
HeLa	Paraquat	HuR	Y	Endog WT	[95]
		HuR	Y	Endog WT	[96]
		HuR/TIAR	Y	Endog WT	[97]
		HuR	Y	Endog WT	[98]
HeLa	Arsenite	HuR	Y	Endog WT	[95]
		HuR	Y	Endog WT	[96]
		HuR	Very Rare	Endog WT	[99]
		HuR	Y	Endog WT	[98]
		TIA1	Y	Endog WT	[100]
		TIAR/G3BP	Y	Endog WT/Exog HA-WT	[30]
		TIA1/G3BP1	Y	Endog WT	[101]
		TIA1	Y	Endog WT	[102]
		TIA1	Y	Exog V5-TDP WT/NLS	[86]
		G3BP1	Y	Exog WT, NLS, G348C	[103]
HeLa	Heat shock	TIA1	Y	Endog WT	[102]
		G3BP1	Y	Exog WT, NLS, G348C	[103]
		TIA1	Y	Exog V5-TDP WT/NLS	[86]
HeLa	Thapsigargin	TIA1	Y	Endog WT	[102]
		HuR	Y	Endog WT	[99]
HeLa	Clotrimazole	TIA1	Y	Exog V5-TDP WT/NLS	[86]
Hek293T	Sorbitol	TIAR/HuR	Y	Endog WT	[104]
		eIF3/TIAR	Y	Endog WT	[105]
		TIAR	Y	Endog WT	[106]
Hek293T	Arsenite	TIAR	N	Endog WT	[104]
		FMRP	Y	Exog NLS	[88]
		eIF3/TIAR	Y	Endog WT	[105]
Hek293T	H2O2	TIAR	Y	Exog WT	[107]
U2OS	Arsenite	eIF3η	Y	Endog WT	[108]
		TIAR	Y	Endog WT	[109]
U2OS	Optogenetic	Opto-G3BP/A11	Y	Endog WT (also pTDP)	[109]
U2OS	Arsenite	G3BP/TIAR	Y†	Endog WT	[110]
SH-SY5Y	Arsenite	G3BP	Y	Exog WT/G348C/NLS	[103]
SH-SY5Y	Heat shock	G3BP	Y	Exog WT/G348C/NLS	[103]
SH-SY5Y	Paraquat	HuR	Y	Endog WT	[95]
		HuR	Y	Endog WT	[97]
		HuR	Y	Endog WT	[96]
		HuR/TIAR	Y	Endog WT	[98]
		HuR	Y	Endog WT	[111]
U87MG	Paraquat	HuR	Y	Endog WT	[95]
astroglia
BE(2)-M17	Arsenite	TIA1	Y	Exog eGFP-WT/Q331K/Q343R	[85]
neuroblastoma
NSC34	Arsenite	TIAR/HuR	Y	Endog WT	[81]
		HuR	Y	Endog WT	[91]
NSC34	Heat shock	TIAR/HuR	Y	Endog WT	[81]
H4 neuro-	Arsenite	G3BP1	Y	Exog GFP-NLS	[82]
glioma
H4 neuro-	Thapsigargin	G3BP1	Y	Exog GFP-NLS	[82]
glioma
Primary Glia	Sorbitol	TIAR/HuR	Y	Endog WT	[104]
Primary	Arsenite	TIAR	Y	Exog GFP-WT	[94]
neurons
Primary	Heat shock	TIA1	Y	Exog V5-TDP WT/NLS	[86]
neurons
iPSC	Optogenetic induction	Opto-G3BP	Y	Endog WT (also pTDP)	[109]
neurons
iPSC	Arsenite	TIA1	Y	Endog WT	[109]
neurons		TIAR	Y (chronic only)	Endog WT and A382T	[90]
iPSC	Heat shock	TIA1	Y	Endog WT	[109]
neurons
iPSC motor	Puromycin	G3BP1	Y	Endog WT/N325S	[82]
neurons
Mouse brain	Arsenite	TIAR	Y	Endog WT	[112]
slices