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An overview of the platforms discussed in this review. The biosynthesis of bicyclic peptides is highlighted in yellow (FIT: flexible in vitro translation; RaPID: random nonstandard peptides integrated discovery; RiPPs: ribosomally synthesized and post-translationally modified peptides; SICLOPPS: split-intein circular ligation of peptides and proteins; NRPS: non-ribosomal synthetase; TE domain: thioesterase domain).
