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Cryo-EM images. (A) Gal-1 alone (0.5 mg/mL); (B) ligand 4 alone (0.17 mg/mL); (C) Gal-3 alone (0.5 mg/mL); (D) Gal-3 + ligand 4 (state concentration); (E) Gal-1 + ligand 4 (state concentration). Scale bar = 100 nm. The NMR lectin/ligand ratios were maintained.
