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The fluorescence resonance energy transfer (FRET)-based mitophagy biosensor.Mito-SRAI (signal-retaining autophagy indicator) consists a sable cyan FP TOLLES (TOLerance of Lysosomal EnvironmentS) and a yellow FP YPet, as a FRET pair, and strict targeting sequences to mitochondria. Because TOLLES is stable to pH changes and lysosomal degradation, this FRET-based mitophagy sensor allows for accurate monitoring of mitophagy process in live and fixed cells.
