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MTT assay for organoid viability in a TNFα-enriched condition. MTT assays were performed in triplicate using jejunal and ileal organoids derived from controls and CD patients (n = 3 each). The optical density (OD) of organoid-containing wells is expressed in a ratio based on the values of the TNFα-free control organoid-containing wells. Differences were evaluated using ANOVA with Bonferroni’s multiple comparison test; * p < 0.05, *** p < 0.001 and **** p < 0.0001.
