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. 2021 Jun 2;26(11):3353. doi: 10.3390/molecules26113353

Figure 1.

Figure 1

Fisetin rescues neuronal cells from oxidative insults. (a) SH-SY5Y cells were treated or not with 2.5 µM of fisetin for 6 h, and cell viability was assessed using CellTiter Blue® Cell Viability Assay kit. (b) SH-SY5Y cells were pretreated or not with 2.5 µM of fisetin for 6 h and then challenged with 35 µM of t-BHP for 18 h. (c) LUHMES cells were treated or not with fisetin (1.25 or 2.5 µM) for 24 h, and cell viability was assessed using CellTiter Blue® Cell Viability Assay kit. (d) LUHMES cells were pretreated or not with fisetin (1.25 or 2.5 µM) for 24 h and then challenged with 5 µM of MPP+ for 24 h. Cell viability was assessed using CellTiter Blue® Cell Viability Assay kit. (e) Tyrosine hydroxylase (TH), (f) dopamine transporter (DAT), and (g) α-synuclein (SNCA) mRNA levels in LUHMES were assessed by qRT-PCR using HPRT1, GAPDH, and B2M as reference genes. The values represent the mean ± SEM of at least three biological replicates. Statistic differences are denoted by * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. indicated condition.