Figure 2.

Fisetin protects against αsyn-induced growth impairment and toxicity. Control cells and cells expressing αsyn–GFP were pre-grown in raffinose medium until mid-log phase. The medium was discarded, and cells were incubated in galactose medium (αsyn ON) supplemented or not with fisetin for 24 h at 30 °C. OD_{600 nm} was monitored hourly. (a) Final biomass 95% confidence intervals were obtained by data modulation using nonlinear parametric regressions and were estimated from the best fit model using RStudio. The vertical dashed line represents the limits of the 95% confidence intervals. (b) The area under the curve (AUC) was integrated using the Origin software (OriginLab, Northampton, MA, USA). (c) Growth curve of control cells, αsyn–GFP-overexpressing cells, and αsyn–GFP-overexpressing cells incubated with the protective concentration of fisetin (10 µM), of which a representative image of the three biological replicates is shown. (d) Flow cytometry analysis of yeast cells incubated with propidium iodide to stain dead cells. Control cells incubated with 10 and 30 μM of fisetin and (e) cells overexpressing αsyn–GFP incubated with 10 and 30 μM of fisetin compared with control cells. The values represent the mean ± SEM of at least three biological replicates. Statistic differences are denoted by * p < 0.05 and *** p < 0.01 vs. indicated condition.