Figure 3.

Cell proliferation and intracellular Ca²⁺ concentrations in renal epithelial cells from Pkd1^+/+ and Pkd1^−/− mice. (A) Analysis of tubular epithelial cell proliferation in kidney sections from male and female Pkd1^+/+ and Pkd1^−/− mice as indicates by Ki-67 staining. Bars = 50 µm. (B) Summary of Ki-67 positive cells/mm² tissue area in kidneys from male and female Pkd1^+/+ and Pkd1^−/− mice. Bars = 50 µm. (C,D) Intracellular basal Ca²⁺ concentrations ([Ca²⁺]_i) as assessed by Fura2 indicates higher basal [Ca²⁺]i_I in male Pkd1^−/− mice. Mean ± SEM (number of animals/number of experiments in each series). ^# Significant difference when compared to Pkd1^+/+ (p < 0.05; unpaired t-test). ^§ Significant difference when compared to male (p < 0.05; ANOVA and Tukey’s post-hoc test).