Figure 7.

Effect of dihydrotestosterone and estrogen on intracellular Ca²⁺ concentrations and expression of Ca²⁺-regulating proteins in mouse mCCDcl1 cortical collecting duct cells. (A) Summary time course of the effects of extracellular Ca²⁺-free buffer and cyclopiazonic acid (10 µM) on intracellular Ca²⁺ concentrations in control mCCDcl1 cells and mCCDcl1 cells treated with dihydrotestosterone (DHT; 10 µM, 24 h) and estrogen (EST, 10 µM; 24 h). Summary of basal intracellular Ca²⁺ concentration and changes in intracellular Ca²⁺ induced by CPA (Ca²⁺ store release through leakage channels; Store) and re-addition of Ca²⁺ to the extracellular buffer (store operated Ca²⁺ entry; SOCE). (B) RT-PCR analysis of the expression of Ca²⁺-regulating proteins and effects of DHT and EST. Mean ± SEM (number of experiments in each series). ^# Significant increase when compared with con (p < 0.05; ANOVA).