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. 2021 Jun 12;26:28. doi: 10.1186/s11658-021-00261-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — β-Elemene suppressed ILK/Akt pathway by affecting the MIR143HG/miR-1275/ILK axis in PHAGF. a The expression level of MIR143HG and miR-1275 was analyzed by RT-qPCR in PHAF, PHAGF, and PHAGF treated with β-elemene. b, c RT-qPCR confirmed the transfection efficiency of pcDNA3.1-MIR143HG, miR-1275 mimics or miR-1275 inhibitors. d, g RT-qPCR was used to detect the ILK/Akt pathway-related mRNAs in PHAGF under indicated treatment. e–f, h–i Western blotting was used to detect expression of ILK/Akt pathway-related proteins in PHAGF under indicated treatment. ^*P < 0.05, ^#P < 0.05, ^##P < 0.01, ^&P < 0.05, versus solvent control group (DMSO) or NC group (negative control)