Skip to main content
. 2021 Jun 3;22(11):6033. doi: 10.3390/ijms22116033

Figure 3.

Figure 3

V600EBRAF inhibition increases autophagy through AMPK-ULK1 signalling. Expression levels of phosphorylated ULK1 at Ser555 (p-ULK), p62 and LC3 (left blots), and quantitative analysis of LC3-II/LC3-I ratios (right graphs) in 8505C and BHT101 cells treated with DMSO (-) or PLX4720 (PLX), in the absence or presence of Dorsomorphin (Dorso) (A) or Rapamycin (Rapa) (B) for 24 h. For each experiment, membranes were reprobed with anti-β-Tubulin as a loading control. Graphic bars represent the LC3-II/LC3-I ratio, calculated after quantitation of LC3-II and LC3-I bands of the blots, and are presented as fold induction relative to the untreated cells. Blots are from one representative experiment and data shown represent the mean ± SEM of the quantitation of at least three independent experiments performed with similar results. Significant differences compared to the corresponding controls: * 0.01 < p < 0.05, ** 0.001 < p < 0.01, *** p < 0.001, compared to untreated cells; # 0.01 < p < 0.05, ### p < 0.001, compared to Dorsomorphin or Rapamycin, respectively.