Figure 5.

Autophagy inhibition enhances antitumor activity of PLX4720 BRAFi in vivo. Mice bearing subcutaneous BHT101 cells-derived xenografts on both flanks were randomly divided into four groups and treated intraperitoneal with vehicle (control), 25 mg/kg/day PLX4720 (PLX), 60 mg/kg Chloroquine (CQ) or combination of both (PLX + CQ) for 12 days. (A) Curves of tumour growth monitored by measuring the tumour volumes over time after the treatments. Data are shown as the mean ± SEM; n = 6. (B) Representative images showing mice from different treatment groups with tumours on day 12 of treatment. Significant differences compared to the corresponding untreated control: * 0.01 < p < 0.05, ** 0.001 < p < 0.01, *** p < 0.001.