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. 2021 Jun 3;26(11):3395. doi: 10.3390/molecules26113395

Table 1.

Detailed information of primers used for the construction of recombinant plasmids expressing SOD1, SOD1-Tat, SOD1-EGFP, or SOD1-EGFP-Tat.

Vector Name Primer Name Primer Sequence Enzyme Site
pET14b SOD1 Forward: 5′ ATAGATACATATGATGGCGATGAAAGCGGTGTGCG 3′ Nde I
Reverse: 5′ TAGATGGTACCCTGCGCAATCCCAATCACTCCAC 3′ Kpn I
pET14b EGFP Forward: 5′ ATTAGGTACCGTGAGCAAGGGCGAGGAGCTGTTC 3′ Kpn I
Reverse: 5′ ATATCTCGAGCTTGTACAGCTCGTCCATGCCGAGA 3′ Xho I
pET14b Tat Forward: 5′ GATCCTATGGCCGCAAGAAACGCCGTCAGCGTCGCCGTG 3′ BamH I
Reverse: 5′ GATCCACGGCGACGCTGACGGCGTTTCTTGCGGCCATAG 3′ BamH I