Table 1.
Vector Name | Primer Name | Primer Sequence | Enzyme Site |
---|---|---|---|
pET14b | SOD1 | Forward: 5′ ATAGATACATATGATGGCGATGAAAGCGGTGTGCG 3′ | Nde I |
Reverse: 5′ TAGATGGTACCCTGCGCAATCCCAATCACTCCAC 3′ | Kpn I | ||
pET14b | EGFP | Forward: 5′ ATTAGGTACCGTGAGCAAGGGCGAGGAGCTGTTC 3′ | Kpn I |
Reverse: 5′ ATATCTCGAGCTTGTACAGCTCGTCCATGCCGAGA 3′ | Xho I | ||
pET14b | Tat | Forward: 5′ GATCCTATGGCCGCAAGAAACGCCGTCAGCGTCGCCGTG 3′ | BamH I |
Reverse: 5′ GATCCACGGCGACGCTGACGGCGTTTCTTGCGGCCATAG 3′ | BamH I |