EVs as a diagnostic tool in clinical practices. UC: Ultracentrifugation, DG: Differential Gradient Centrifugation, DC: Differential centrifugation, SEC: Size Exclusion Chromatography, TEM: Transmission Electron Microscopy, NTA: Nanoparticle Tracking Analysis, FC: Flow Cytometry, qRT-PCR: quantitative Real Time Polymerase Chain Reaction, PB: Peripheral Blood, CSF: Cerebrospinal fluid, PE: Pleural effusion. This figure has been created by Emilio Venturelli using Servier Medical Art templates, which are licensed under a Creative Commons Attribution 3.0 Unported License; https://smart.servier.com (accessed on 14 April 2021) [11].