Skip to main content
. 2021 Jun 4;13(11):2804. doi: 10.3390/cancers13112804

Figure 7.

Figure 7

Direct regulation of ITGA6 and ITGB1 expression by miR-29-3p in ICC cells. (A) Dual-luciferase reporter assays showed that luminescence activity was reduced by co-transfection with wild-type vector (containing the miR-29-3p binding site of ITGA6) and miR-29c-3p precursor in HuCCT1 and RBE cells. In contrast, no change of luminescence activity occurred after co-transfection with deletion-type vector luciferase activity with miR-29-3p transfection into ICC cells. Normalized data were calculated as the ratios of Renilla/firefly. (B) The luminescence activity was reduced by co-transfection with wild-type vector (containing miR-29-3p binding site of ITGB1) and miR-29c-3p precursor in HuCCT1 and RBE cells. In contrast, no change of luminescence activity occurred after co-transfection with deletion-type vector luciferase activity.