Figure 13.

Direct regulation of SP1 by miR-29-family in intrahepatic cholangiocarcinoma (ICC) cells. (A) The luminescence activity was reduced by co-transfection with wild-type vector (containing miR-29-3p binding site of SP1) and miR-29c-3p precursor in HuCCT1 and RBE cells. In contrast, no change of luminescence activity occurred after co-transfection with deletion-type vector luciferase activity. (B) Expression levels of SP1 were significantly reduced by miR-29a-3p, miR-29b-3p and miR-29c-3p transfection into ICC cells. GUSB was used as an internal control. (C) Protein expression levels of SP1 were significantly reduced by miR-29a-3p, miR-29b-3p, and miR-29c-3p transfection into ICC cells (72 h after transfection). GAPDH was used as an internal control.