Figure 5.

Rluc8-fused monobodies efficiently bound cancer cells with exposed ecto-CRT. Cancer cells (CT-26 and MC-38) were treated with anticancer agents (DOX, MTX, or GEM) for 4 h. Then, cells were stained with monobodies and analyzed by flow cytometry. (A,C) Flow cytometry of CT-26 cells. (B,D) Flow cytometry of MC-38 cells. PBS as negative control. Second Ab is used as control of the background fluorescence signals. The increase in MFI is shown relative to the respective control groups. Data show the mean ± standard error (n = 3). *** p < 0.001, and ns = non-significant.