Figure 6.

Immunofluorescence imaging analysis of the binding of Rluc8-fused monobodies to ecto-CRT in cancer cells treated with anticancer drugs. CT-26 and MC-38 cells treated with anticancer agents for 4 h were stained with ecto-CRT monobodies (CRT-3-Rluc8, CRT-4-Rluc8, and #DGR-Rluc8) and a secondary antibody (green) and observed by confocal microscopy (40×, magnification). The cell membranes were stained with fluorochrome-labeled wheat germ agglutinin (WGA) (red). Nuclei was stained with DAPI (blue). Scale bar represents 50 µm.