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Stability of Rluc8-fused monobodies in serum. The monobodies were mixed with mouse serum and incubated at 37 °C for the indicated times. The luciferase bioluminescence activity of the monobodies was measured with an IVIS imaging system after adding coelenterazine (40 µg/mL). Data represent the mean ± standard error (n = 3).
