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. 2021 Jun 4;22(11):6079. doi: 10.3390/ijms22116079

Figure 5.

Figure 5

Evaluation of the specificity of AAL binding to fuco-SERPINA1 in reverse lectin ELISA. (a) Different amounts of recombinant SERPINA1 protein were treated with or without PNGase F (PNG) (protein:PNGase F = 1 µg:1U, 37 °C for 20 h) and subjected to AAL-based reverse lectin ELISA. (b) AAL was pre-incubated with different doses of L-fucose or lactose at room temperature for 0.5 h and coated onto ELISA plates. Plates were subjected to reverse lectin ELISA for detecting fixed amounts of fuco-SERPINA1. OD values were measured at 450 nm and each data point was examined in duplicate. Data expressed as mean ± S.D. are presented as a black solid line.