Table 2.
Numbers of identified proteins, peptides and glycopeptides in plasma proteome and glycoproteome.
| Identified Proteins, Peptides and Glycopeptides | Quantitative Proteome Profiling | Quantitative Glycoproteome Profiling | ||||
|---|---|---|---|---|---|---|
| GS + M0 + M1 | M0/GS | M1/GS | GS + M0 + M1 | M0/GS | M1/GS | |
| Total proteins | 1707 | 1489 | 1472 | 2160 | 1749 | 1953 |
| N to D_18O proteins | - | - | - | 145 | 133 | 130 |
| Total peptides | 10,102 | 8621 | 8304 | 10,572 | 8453 | 8872 |
| N to D_18O peptides | - | - | - | 333 | 281 | 267 |
| Ratio (N to D_18O peptides/total peptides) | - | - | - | 3.15% | 3.32% | 3.01% |
| N to D_18O and NXS/T/C peptides | - | - | - | 284 | 244 | 232 |
| Ratio (N to D_18O and NXS/T/C peptides/N to D_18O peptides) |
- | - | - | 85.29% | 86.83% | 86.89% |
N to D_18O, asparagine (N) was transferred to aspartate (D) by replacing -NH2 with -18OH via glycosidase treatment (PNGase F) in H218O-containing buffer. NXS/T/C, consensus sequence of N-linkage glycosylation, where X represents any amino acid except proline.