Figure 3. The effect of PGRN on Aβ plaque in the 10-mo-old 5XFAD mice.

(A) Quantification of ThioS-positive and Aβ immunoreactive area, number and intensity in subiculum and cortex of 10-mo-old male and female 5XFAD, 5XFAD Grn^−/− mice. Mean ± SEM, n = 5, t test, *P < 0.05, **P < 0.01. (B) Aβ40 and Aβ42 levels in the RIPA-soluble fraction (soluble) and guanidine-soluble fraction (insoluble) from 10-mo-old male and female 5XFAD, 5XFAD Grn^−/− mice were measured by ELISA. Mean ± SEM, n = 4–5, t test.