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. 2021 Jun 8;4(7):e202000874. doi: 10.26508/lsa.202000874

Figure 5. PGRN deficiency leads to an increase in the levels of microglial glycoprotein nonmetastatic melanoma protein B (GPNMB) in the 5XFAD mice.

Figure 5.

(A, B, C, D) Western blot analysis of GPNMB in the cortical lysates from 4.5-mo-old (A, B) and 10-mo-old (C, D) WT, Grn−/−, 5XFAD and 5XFAD Grn−/− mice. Female 4.5-mo-old 5XFAD and 5XFAD Grn−/− mice were used. For other groups, mice of mixed sex were used. Mean ± SEM; n = 3, one-way ANOVA, *P < 0.05, **P < 0.01. The same samples were used for experiments in Figs 4A–D and 5A–D and the experiments were performed simultaneously. (E) Immunostaining of IBA1, GPNMB, and Aβ (6E10) in the brain sections from 4.5-mo-old female 5XFAD and 5XFAD Grn−/− mice. Representative images from the cortex region were shown. Scale bar = 100 µm. Representative high-resolution confocal images were shown in inset. Scale bar = 10 µm. (E, F) Quantification of GPNMB levels in IBA1-positive microglia in the cortex region for experiment in (E). Mean ± SEM; n = 6, t test, **P < 0.01. (G) Representative confocal high-resolution images of GPNMB and galectin-3 co-staining in the brain sections from 4.5-mo-old 5XFAD and 5XFAD Grn−/− mice. Scale bar = 10 µm.