Figure 8. PGRN deficiency leads to an up-regulation of cathepsin B in the microglia near Aβ plaque in the 5XFAD mice.

(A) Immunostaining of IBA1, cathepsin B (CathB), and Aβ (6E10) in the brain sections from 4.5-mo-old female 5XFAD and 5XFAD Grn^−/− mice. Representative images from the cortex region were shown. Scale bar = 100 µm. (A, B) Quantification of CathB levels near Aβ plaque for experiment in (A). Mean ± SEM; n = 3, t test, **P < 0.01. (C) Representative confocal high-resolution images of IBA1, CathB, and Aβ (6E10) co-staining in the brain sections from 4.5-mo-old 5XFAD and 5XFAD Grn^−/− mice. Scale bar = 10 µm. (D) Representative confocal high-resolution images of galectin-3, CathB, and IBA1 co-staining in the brain sections from 4.5-mo-old 5XFAD and 5XFAD Grn^−/− mice. Scale bar = 10 µm.