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. 2021 Jun 11;4(7):e202101038. doi: 10.26508/lsa.202101038

Figure 3. Nuclear localization of WBP2 modifies its capability in regulating the microprocessor complex.

Figure 3.

(A) Analysis of nuclear export signal (NES)- and NLS-tagged WBP2 localization in MCF-7 cells using immunofluorescence method (n = 10 cells). (B) NLS-WBP2 localizes significantly in the cytoplasm, whereas NES-WBP2 localizes in the nucleus. (C) NES-GFP-WBP2 is unable to inhibit the microprocessor complex activity when the endogenous WBP2 is depleted using pri-miR-205 construct–based microprocessor complex assay in MCF-7 cells. Whereas NLS-GFP-WBP2 significantly inhibits the microprocessor complex greater than wild-type-GFP-WBP2. (D) The wild-type/NES/NLS-GFP–tagged exogenous WBP2 are overexpressed properly, whereas the endogenous WBP2 is silenced. DGCR8 is overexpressed as a positive control of the microprocessor complex activity assay.