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. 2021 Jun 11;4(7):e202101038. doi: 10.26508/lsa.202101038

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© 2021 Tabatabaeian et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 4. — (A) WBP2 overexpression (i) or depletion (ii) do not affect the expression level of DGCR8, Drosha, DDX5, and DDX17 in MCF-7 and T47D cells. (B-i) Analysis of WBP2, DGCR8, DDX5, and DDX17 expression levels in the cytoplasm and nucleus of MCF-7 and T47D cells. (B-ii) Analysis of WBP2’s interaction with the microprocessor complex components in subcellular fractionations of MCF-7 and T47D cells, upon WBP2 pull-down. (C) Reciprocal analysis of WBP2’s interaction with the microprocessor complex components in subcellular fractions of MCF-7 cells, upon DGCR8, Drosha, DDX5, or DDX17 pull-down. (D) Reciprocal analysis of WBP2’s interaction with the microprocessor complex components in subcellular fractions of T47D cells, upon DGCR8, Drosha, DDX5, or DDX17 pull-down.