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. 2021 Mar 5;12(3):717–730. doi: 10.1002/jcsm.12693

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© 2021 The Authors. Journal of Cachexia, Sarcopenia and Muscle published by John Wiley & Sons Ltd on behalf of Society on Sarcopenia, Cachexia and Wasting Disorders.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Immunoblot data for marks of protein synthesis in males and females across different durations of unloading. (A) Akt protein content in the extensor digitorum longus (EDL) of males and females across different durations of unloading. (B) Akt protein content in the gastrocnemius of males and females across different durations of unloading. (C) pAkt^Ser473 protein content in the EDL of males and females across different durations of unloading. (D) pAkt^Ser473 protein content in the gastrocnemius of males and females across different durations of unloading. (E) pAkt^Ser473:Akt protein ratio in the EDL of males and females across different durations of unloading. (F) pAkt^Ser473:Akt protein ratio in the gastrocnemius of males and females across different durations of unloading. (G) 4EBP1 protein content in the EDL of males and females across different durations of unloading. (H) 4EBP1 protein content in the gastrocnemius of males and females across different durations of unloading. (I) p4EBP1^Thr37/46 protein content in the EDL of males and females across different durations of unloading. (J) p4EBP1^Thr37/46 protein content in the gastrocnemius of males and females across different durations of unloading. (K) p4EBP1^Thr37/46:4EBP1 protein ratio content in the EDL of males and females across different durations of unloading. (L) p4EBP1^Thr37/46:4EBP1 protein ratio content in the gastrocnemius of males and females across different durations of unloading. (M) Representative images for EDL immunoblot data in males. (N) Representative images for EDL immunoblot data in females. (O) Representative images for gastrocnemius immunoblot data in males. (P) Representative images for gastrocnemius immunoblot data in females. Different letters represent statistical differences at Tukey‐adjusted P ≤ 0.05. *Linear trend within a sex. ^ΩQuadratic trend within a sex. ^#Cubic trend within a sex. Female data are italicized and underlined.