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. 2021 May 6;12(3):746–768. doi: 10.1002/jcsm.12700

Figure 3.

Figure 3

Tfr1 ablation in satellite cells (SCs) delays skeletal muscle regeneration. (A) Timeline characterizing skeletal muscle regeneration upon tamoxifen‐induced Tfr1 ablation in SCs. (B) Representative image of transverse abdominal (TA) muscle upon completion of regeneration between Tfr1 SC/WT and Tfr1 SC/KO mice at 30 dpi. (C) Summary of body weight, TA muscle weight, and ratio of TA muscle and body weight between Tfr1 SC/WT and Tfr1 SC/KO mice upon completion of regeneration at 30 dpi (n = 7 per group). (D) Representative images of TA muscle section from Tfr1 SC/WT and Tfr1 SC/KO mice (n = 6 mice per group). Immunofluorescence of Pax7 revealed a decrease in the number of Pax7+ SCs (arrowheads) and number of Pax7+ SCs per TA muscle section at 5 dpi (right of immunostaining images). (E) Representative images of TA muscle section from Tfr1 SC/WT and Tfr1 SC/KO mice (n = 6 mice per group). Immunofluorescence of Pax7 revealed a decrease in the number of Pax7+ SCs (arrowheads) and number of Pax7+ SCs per TA muscle section at 9 dpi (right of immunostaining images). (F) Immunofluorescence of eMyHC+ myotubes after cardiotoxin (CTX) injury (5 dpi) and number of eMyHC+ myotubes per TA muscle section area at 5 dpi (right of immunostaining images). (G) Immunofluorescence of eMyHC+ myotubes after CTX injury (5 dpi) and number of eMyHC+ myotubes per TA muscle section area at 9 dpi (right of immunostaining images). (H) Immunofluorescence of Pax7 revealed a decrease in the number of Pax7+ SCs (arrowheads) and number of Pax7+ SCs per TA muscle section at 30 dpi (right of immunostaining images, n = 6 mice per group). (I) Representative images of TA muscles from Tfr1 SC/WT and Tfr1 SC/KO mice with haematoxylin and eosin staining and Masson staining upon completion of CTX‐induced regeneration (30 dpi, n = 6 mice per group). (J) Summary of collagen volume fraction between Tfr1 SC/WT and Tfr1 SC/KO mice completion of CTX‐induced regeneration at 30 dpi. N.S., not significant, *P < 0.05, **P < 0.01, and ***P < 0.005, by two‐sided Student's t‐test. Data represent the mean ± standard error of the mean.