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. 2021 May 6;12(3):746–768. doi: 10.1002/jcsm.12700

Figure 6.

Figure 6

Ferroptosis in transverse abdominal (TA) muscle of Tfr1 SC/KO mice prevents skeletal muscle regeneration. (A) Timeline characterizing the activation of ferroptosis in TA muscle of Tfr1 SC/WT and Tfr1 SC/KO mice after cardiotoxin (CTX)‐induced injury. (B) qPCR analysis of Gpx4 and Ptgs2 expression in TA muscle from adult Tfr1 SC/WT and Tfr1 SC/KO mice after CTX‐induced injury at 5, 9, and 15 dpi. (C) Representative images of TA muscle section with Prussian blue and Oil Red O (ORO) staining from Tfr1 SC/WT and Tfr1 SC/KO mice after CTX‐induced injury at 5 and 9 dpi. (D) Timeline characterizing the effect of ferrostatin‐1 to inhibit ferroptosis in TA muscle from Tfr1 SC/WT and Tfr1 SC/KO mice. (E) Representative images of TA muscle section immunostaining for Pax7 (red) and laminin B2 (white). Number of Pax7+ SCs per section (right) (n = 6 per group). (F) Representative images of TA muscle section with Prussian blue and ORO staining from adult Tfr1 SC/WT and Tfr1 SC/KO mice after CTX‐induced injury between saline and ferrostatin‐1 intraperitoneal injection at 30 dpi. (G) Timeline characterizing the effect of lenti‐Tfr1 to inhibit ferroptosis in TA muscle from Tfr1 SC/WT and Tfr1 SC/KO mice. (H) Representative images of TA muscle section immunostaining for Pax7 (red) and laminin B2 (white) between lenti‐Ctrl and lenti‐Tfr1 intramuscular injection at 30 dpi. Number of Pax7+ SCs per section (right) (n = 6 per group). (I) Representative images of TA muscle section with Prussian blue and ORO staining from adult Tfr1 SC/WT and Tfr1 SC/KO mice after CTX‐induced injury between lenti‐Ctrl and lenti‐Tfr1 intramuscular injection at 15 dpi. (J) Respiratory exchange rate (VCO2/VO2) and energy expenditure were monitored over a 48 h period for Tfr1 SC/WT and Tfr1 SC/KO mice after CTX‐induced injury at 15 dpi (n = 8 mice per group). (L) Representative transmission electron microscope image of TA muscle samples from adult Tfr1 SC/WT and Tfr1 SC/KO mice after CTX‐induced injury at 30 dpi. N.S., not significant, *P < 0.05, and ***P < 0.005, by two‐sided Student's t‐test. Data represent the mean ± standard error of the mean.