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. 2021 Jun 9;14:3671–3685. doi: 10.2147/OTT.S299190

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© 2021 Qi et al.

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Overexpression of miR-326 partially reversed the effect of circHIPK3 on apoptosis and epithelial–mesenchymal transition-related genes. (A–C) The expressions of apoptosis-related genes (cleaved Caspase-3, Bcl-2 and Bax) in NC + MC, circHIPK3+ MC, NC + M, and circHIPK3 +M groups were detected by Western blot and RT-qPCR. (D–F) The expressions of E-Cadherin, N-Cadherin and vimentin in NC + MC, circHIPK3+ MC, NC + M, and circHIPK3 +M groups were detected by Western blot and RT-qPCR. Each experiment was repeated three times independently. GAPDH was set as control. *P < 0.05, **P < 0.01, ***P < 0.001 vs NC + MC; ^{^}P < 0.05, ^{^^}P < 0.01, ^{^^^}P < 0.001 vs circHIPK3 + MC; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs NC + M.