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. 2021 May 31;8:621273. doi: 10.3389/fnut.2021.621273

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Copyright © 2021 Naz, Okla, Fatima, Mohsin, Soufan, Alaraidh, Abdel-Maksoud and Ahmad.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NeuAc nanosensor. (A) Open form of SiaP protein, (B) Closed form of SiaP protein (26, 27), (C) Diagram represents the restriction sites of ECFP-SiaP-Venus construct, (D) General demonstration of working of FLIP-SA. Addition of NeuAc with the sensory domain (SiaP) brings the ECFP and Venus closure, resulting the FRET.