Table 3.
Prediction analysis of the functional impact of miRNA variants associated with AMD.
| MiRNA | SNP (alleles) | Risk allele | MFE (allele) | Disrupted target sites by the variant allele | Created target sites by the variant allele | AMD-associated genes targeted by miRNAs | Biological pathways |
|---|---|---|---|---|---|---|---|
| MiR-196a2-3p | rs11614913 (C/T) | C | MFE (C): −50.30 kcal/mol MFE (T): −44.70 kcal/mol |
na | Na | MAPK1, CD58, IL2RA, IL23R | Neuroinflammation, neurodegeneration, oxidative stress, endoplasmic reticulum stress, synaptogenesis, endocytosis, unfolded protein response |
| MiR-196a2-5p | - | na | Na | ATF6, MAPK1, TNFSF14 | |||
| MiR-6796-3p | rs3745198 (C/G) | C | MFE (C): −32.30 kcal/mol MFE (G): −28.50 kcal/mol |
- |
CYP24A1, MAPK1 |
ARMS2, ATF6, CD58, MAPK1, TNFSF14 | Cell motility, cell differentiation, signal transduction, neurogenesis, neuron differentiation, axon development, angiogenesis |
| MiR-6796-5p | - | - | - | APOE, ATF6, CLECL1. INPP5D, MAPK1, TNFSF14, ZSWIM4 | |||
| MiR-6499-3p | rs3734050 (C/T) | C | - | - | - | ARMS2, ATF6, CD58, CYP24A1, IL2RA, IL23R, MAPK1, TNFSF14, ZSWIM4 | Mitochondrial function, vesicle trafficking, cell motility, signal transduction, regulation of cell death, neurogenesis, neuron differentiation, endoplasmic reticulum stress |
| MiR-6499-5p | MFE (C): −24.50 kcal/mol MFE (T): −25.20 kcal/mol |
- | - | - | |||
| MiR-6810-3p | rs62182086 (A/G) | A | - | - | - | ATF6, MAPK1, TNFSF14, ZSWIM4 | Synaptogenesis signaling, neurogenesis, differentiation and projection of neurons, signal transduction |
| MiR-6810-5p | MFE (A): −30.20 kcal/mol MFE (G): −30.40 kcal/mol |
INPP5D | TNFSF14 | APOE, ARMS2, ATF6, CD58, IL23R, INPP5D, MAPK1, TNFSF14 | |||
| MiR-499a-3p | rs3746444 (A/G) | G | MFE (A): −63.20 kcal/mol MFE (G): −62.80 kcal/mol |
INPP5D | - | ATF6, CD58, MAPK1, INPP5D, TNFSF14 | Neuroinflammation, neurodegeneration, oxidative stress, endoplasmic reticulum stress, synaptogenesis, endocytosis, unfolded protein response |
| MiR-499a-5p | - | - | - | ATF6, MAPK1 | |||
| MiR-7854-3p | rs2925980 (A/G) | G | MFE (A): −26.40 kcal/mol MFE (G): −25.50 kcal/mol |
- | CD58, ZSWIM4, ATF6 | ATF6, CD58, CYP24A1, MAPK1, TNFSF14, ZSWIM4 | Cell motility, cell differentiation, signal transduction, vesicle trafficking, synaptogenesis signaling |
| MiR-7854-5p | - | - | - | - |
MFE, minimum free energy; AMD, age-related macular degeneration.