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. 2021 May 31;15:628917. doi: 10.3389/fnins.2021.628917

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Copyright © 2021 Zou, Guo, Zhu, Xu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mouse traumatic spinal cord injury. (A) Laminectomy was performed at the T9-10 vertebrate, and the exposed spinal cord (T10) was impacted with a 5-gram rod dropped 12.5 mm by NYU Impactor. Following removal of the rod, the muscles and skin were sutured in layers. (B) The operation schematic diagram. (C) Gross morphology and representative hematoxylin-eosin staining sagittal sections. Scale bar: 500 μm. N = 10 for each experiment. Sham group, which underwent laminectomy without TSCI; SCI group, which underwent laminectomy followed by TSCI and received saline i.v. immediately after injury; MP group, which underwent laminectomy followed by TSCI and received a 30 mg/kg dose of MP (Pfizer Inc., United States) i.v. immediately after TSCI via the tail vein injection.