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. 2021 May 31;12:672449. doi: 10.3389/fimmu.2021.672449

Figure 2.

Figure 2

Absence of CpG motifs significantly reduce the percentage of AAV capsid specific CD8+ T cells but does not impact capsid-specific antibody formation. (A) Schematic representation of CpG-free expression cassette encoding hFIX with padua mutation. This cassette was packaged into AAV2-SIINFEKL capsid. (B) Experimental timeline of WT C57BL/6 mice injected with either AAV2-SIINFEKL-CpG+ or AAV2-SIINFEKL-CpG− vector. (C) Representative flow plots showing AAV capsid-specific CD8+ T cells at different time points in mice injected with AAV2-SIINFEKL-CpG+ and AAV2-SIINFEKL-CpG− vector. (D) Percentages of capsid specific CD8+ T cell in mice injected with AAV2-SIINFEKL-CpG+ and AAV2-SIINFEKL-CpG− vector. The dotted line at 0.045% represents the limit of detection of capsid specific CD8+ T cells using the tetramer. (E) Anti-AAV2 IgG2c antibody titers in plasma samples from mice injected with AAV2-SIINFEKL-CpG+ or AAV2-SIINFEKL-CpG− vector. Samples were collected at days 14 and 28 post vector injection. Data are average ± SEM of at least five animals per group. Each circle represents an individual animal. Statistically significant differences are indicated. *P < 0.05, ****P < 0.0001.